Used in bioconjugation processes to link biomolecules such as proteins, peptides, or antibodies with targeting agents or detection molecules. The biotin moiety allows strong binding to streptavidin or avidin platforms, commonly used in ELISA, flow cytometry, and diagnostic assays. The PEG3 spacer enhances solubility and reduces steric hindrance, improving binding efficiency. Frequently applied in the development of targeted drug delivery systems, biosensors, and imaging probes where precise molecular recognition is required. Also utilized in solid-phase synthesis and surface functionalization of nanoparticles or microarrays.