Amine-PEG3-Biotin(TFA) is used in bioconjugation processes to link biomolecules with high specificity. The terminal amine group enables coupling to activated carboxyl groups on proteins or other molecules, while the biotin moiety binds strongly to streptavidin or avidin, making it ideal for detection, purification, and immobilization in assays such as ELISA, flow cytometry, and Western blotting. The PEG3 spacer enhances solubility and reduces steric hindrance, improving binding efficiency. Commonly used in probe development, target identification, and pull-down assays in proteomics and molecular biology.