Berbamine dihydrochloride is widely studied for its potential anticancer properties. It has shown activity against various cancer cell lines by inducing apoptosis, inhibiting cell proliferation, and suppressing tumor growth. Research highlights its effectiveness in overcoming multidrug resistance in cancer therapy, making it a candidate for use in combination with conventional chemotherapeutic agents. It also exhibits immunomodulatory effects, influencing T-cell activity and cytokine production, which may benefit autoimmune disease management and immune system regulation. Additionally, berbamine dihydrochloride has demonstrated anti-inflammatory and antioxidant activities, suggesting possible applications in treating inflammatory conditions and oxidative stress-related diseases. Emerging studies explore its role in cardiovascular protection, where it may help reduce myocardial injury and regulate calcium channels in heart cells. Due to its broad biological activity, it is used in preclinical research to investigate new therapeutic strategies, particularly in oncology and immunology.