N-tetradecyl β-D-maltoside is widely used in biochemical research as a non-ionic detergent for solubilizing membrane proteins. Its ability to maintain protein stability and functionality makes it ideal for studying membrane-bound proteins, such as receptors and enzymes, in their native state. It is also employed in the purification and crystallization of membrane proteins, aiding in structural biology and drug discovery. Additionally, it is utilized in the preparation of lipid vesicles and liposomes for drug delivery systems, enhancing the encapsulation and release of therapeutic agents. Its mild detergent properties make it suitable for use in cell lysis and protein extraction processes without denaturing proteins.