Nonylβ-D-Maltopyranoside is widely used in biochemistry and molecular biology for solubilizing membrane proteins. Its ability to effectively extract and stabilize proteins makes it a valuable tool in the study of membrane-bound proteins, ensuring they remain functional and intact during experiments. This chemical is particularly useful in the preparation of protein samples for X-ray crystallography and cryo-electron microscopy, where maintaining protein integrity is crucial. Additionally, it is employed in the purification of membrane proteins, aiding in their isolation from cell membranes without denaturation. Its mild detergent properties also make it suitable for use in various enzymatic assays and reconstitution experiments, providing a stable environment for protein activity.