Used in biological and biochemical research to enhance membrane permeability, allowing better penetration of antibodies, dyes, or other reagents into cells and tissues. Commonly applied in immunostaining, flow cytometry, and histochemistry to improve signal detection by facilitating access to intracellular targets. Its formulation in Tris-buffered saline (TBS) maintains physiological pH and osmolarity, making it compatible with a wide range of assays. Effective for both fixed and live cell applications where controlled permeabilization is required without significant disruption of cellular structure.