Cyclo(L-Pro-L-Val) is primarily used in the field of peptide synthesis and pharmaceutical research. It serves as a key intermediate in the development of bioactive peptides and peptidomimetics, which are essential for creating drugs with enhanced stability and specificity. This compound is also employed in studying enzyme inhibition, particularly in the context of proteases, due to its cyclic structure that mimics natural peptide substrates. Additionally, it finds application in material science for designing peptide-based nanomaterials and biomaterials with tailored properties. Its role in modulating biological pathways makes it valuable for exploring therapeutic targets in diseases like cancer and microbial infections.