Gly-Phe-Arg is a synthetic tripeptide consisting of glycine, phenylalanine, and arginine residues. It is primarily utilized in biochemical and biomedical research as a substrate for enzymatic assays, particularly for studying proteases such as aminopeptidases involved in protein degradation. The peptide's structure allows it to serve as a model compound for investigating cellular signaling, enzyme kinetics, and peptide hydrolysis. Additionally, due to the arginine moiety, it is explored for potential applications in modulating inflammatory responses and vascular functions, such as in studies related to circulatory disorders. Gly-Phe-Arg may also contribute to peptide-based drug development as an intermediate or active component in therapeutic designs.