D-threo Sphinganine (d18:0) is primarily used in research related to sphingolipid metabolism and cell signaling pathways. It serves as a key intermediate in the biosynthesis of more complex sphingolipids, which are essential components of cell membranes and play critical roles in cellular processes such as apoptosis, proliferation, and differentiation.

In biochemical studies, it is utilized to investigate the enzymatic steps involved in sphingolipid synthesis, particularly the activity of serine palmitoyltransferase and ceramide synthase. It is also employed in studies exploring the role of sphingolipids in diseases like cancer, neurodegenerative disorders, and metabolic syndromes.

Additionally, D-threo Sphinganine (d18:0) is used as a standard in analytical techniques such as mass spectrometry and chromatography to quantify and identify sphingolipids in biological samples. Its application extends to the development of therapeutic strategies targeting sphingolipid pathways for disease treatment.