15-keto Prostaglandin F1α is primarily used in research settings to study its role in physiological processes, particularly in inflammation and reproductive biology. It serves as a biomarker for the oxidative metabolism of prostaglandins, helping researchers understand the pathways and mechanisms involved in these processes. In reproductive studies, it is used to investigate its effects on uterine contractions and luteolysis, providing insights into pregnancy and parturition. Additionally, it is utilized in pharmacological studies to explore its potential therapeutic applications in conditions related to inflammation and vascular function. Its role in lipid metabolism and oxidative stress is also a key area of interest in biomedical research.