17-trans Prostaglandin F3α is primarily used in research settings to study its biological effects and mechanisms of action. It is often employed in studies related to reproductive physiology, particularly in understanding its role in uterine contractions and luteolysis, which are critical processes in reproductive health. Additionally, it is investigated for its potential effects on inflammation and vascular responses, providing insights into its therapeutic applications in conditions like cardiovascular diseases and inflammatory disorders. Researchers also explore its interactions with prostaglandin receptors to develop targeted therapies for various medical conditions.