Used in the pharmaceutical industry as a key component in the formulation of liposomes and lipid nanoparticles, especially for drug delivery systems including mRNA vaccines. Acts as a stabilizing agent in membranes, enhancing the integrity and fluidity of liposomal structures. Employed in cell culture media to support the growth of certain mammalian cells. Also utilized in the synthesis of steroid hormones such as cortisol, progesterone, and testosterone.

In cosmetics and skincare products, cholesterol reinforces the natural skin barrier, helping to maintain moisture and reduce transepidermal water loss. It is commonly incorporated into creams, lotions, and formulations for dry or sensitive skin.

Commonly found in research settings to study membrane biology, lipid metabolism, and the flexibility and stability of cell membranes.