Lyso-PAF C-16-d4 is primarily used in biochemical research as a stable isotope-labeled internal standard for the quantification of lyso-platelet-activating factor (lyso-PAF) and related lipids in biological samples. Its deuterated structure ensures accurate and reliable measurements in mass spectrometry-based lipidomics studies, enabling researchers to study lipid metabolism, inflammation, and signaling pathways. It is particularly valuable in investigating the role of lyso-PAF in diseases such as asthma, cardiovascular disorders, and immune responses, providing insights into therapeutic targets and biomarker discovery.