5(S),6(S)-DiHETE is primarily involved in inflammatory and immune responses within the body. It plays a role as a lipid mediator, influencing cellular signaling pathways that regulate inflammation. This compound is particularly significant in studies related to the resolution of inflammation, as it helps modulate the activity of immune cells and promotes tissue repair. Researchers are exploring its potential therapeutic applications in conditions characterized by chronic inflammation, such as autoimmune diseases and cardiovascular disorders. Additionally, it is being investigated for its role in modulating pain and its interactions with other lipid mediators in the eicosanoid pathway.