Used in biomedical research as an internal standard in mass spectrometry for the quantification of 25-hydroxycholesterol in biological samples. It helps in studying cholesterol metabolism, oxysterol signaling, and their roles in various diseases such as atherosclerosis, inflammation, and neurodegeneration. Applied in lipidomics to understand oxysterol profiles and their impact on cellular functions, including regulation of lipid homeostasis and immune responses.