2-Arachidonoyl Glycerol-d8 (2-AG-d8) is primarily used as an internal standard in analytical chemistry for the quantification of 2-arachidonoyl glycerol (2-AG) in biological samples. It is particularly valuable in mass spectrometry and chromatography techniques, such as LC-MS/MS, where it helps ensure accurate and precise measurements by compensating for variations in sample preparation and instrument performance. This deuterated analog is essential in research focused on the endocannabinoid system, aiding in studies related to neurotransmitter regulation, pain management, and inflammatory responses. Its application is critical in understanding the role of 2-AG in various physiological and pathological processes.