Bile Salt No. 3 is widely used in biochemical and medical research due to its role in emulsifying fats and aiding in digestion. It is commonly employed in laboratory settings to study lipid metabolism and the absorption of fat-soluble vitamins. In cell culture, it helps in maintaining the solubility of hydrophobic compounds, ensuring consistent experimental conditions. Additionally, it is utilized in diagnostic assays to simulate physiological conditions for testing liver and gallbladder functions. Its ability to form micelles makes it valuable in drug delivery systems, enhancing the bioavailability of poorly soluble drugs. In the food industry, it is sometimes used as an additive to improve the texture and stability of certain products.