Used in research on bile acid metabolism and liver function, particularly in murine models for studies related to cholestasis and metabolic disorders. Acts as an antagonist of the farnesoid X receptor (FXR), aiding in the regulation of bile acid homeostasis and exploration of FXR-independent pathways. Applied in cell culture and animal models to investigate gut-liver axis signaling, potential therapeutic interventions for liver diseases, and the impact of bile acids on glucose and lipid metabolism. Also utilized in studies examining bile acid interactions with gut microbiota composition.