Sodium cholate hydrate is widely used in biochemical and pharmaceutical research as a bile salt surfactant. It is particularly valuable in solubilizing membrane proteins and lipids, making it essential for studying cellular structures and functions. In drug delivery systems, it enhances the absorption of hydrophobic drugs by improving their solubility in aqueous environments. Additionally, it is employed in diagnostic assays and as a stabilizing agent in various formulations. Its ability to form micelles also makes it useful in nanotechnology for creating nanostructured materials.