This bifunctional chemical is widely used in bioconjugation processes, particularly in the modification of proteins, peptides, and antibodies. The maleimide group enables specific coupling with thiol-containing molecules, while the reactive TFP ester group facilitates efficient conjugation with amine-containing molecules, allowing the creation of stable thioether and amide bonds, respectively. The PEG2 spacer enhances solubility, reduces steric hindrance, and improves the flexibility of the conjugated molecules in biological systems. It is commonly applied in the development of drug delivery systems, diagnostic probes, and targeted therapies, where precise and stable conjugation is critical. Additionally, it is utilized in research for labeling biomolecules to study their interactions, localization, and function in vitro and in vivo.