Dimethyl 2-oxosuccinate is widely used in organic synthesis as a versatile intermediate. It plays a key role in the production of fine chemicals and pharmaceuticals, particularly in the synthesis of heterocyclic compounds. Its structure allows it to participate in various reactions, such as cyclization and condensation, making it valuable for creating complex molecules. Additionally, it is employed in the development of agrochemicals and dyes, where its reactivity contributes to the formation of functionalized products. In research, it serves as a building block for studying reaction mechanisms and designing new compounds.