Used as a fluorescent probe in biochemical assays due to its ability to emit light upon excitation, 6-benzoxazolol is valuable in studying protein interactions and conformational changes. Its derivatives are incorporated into sensors for detecting metal ions such as zinc and copper in environmental and biological samples. It also serves as a building block in synthesizing pharmaceuticals with anti-inflammatory and antimicrobial properties. In materials science, compounds based on 6-benzoxazolol are applied in organic light-emitting diodes (OLEDs) and fluorescent dyes for imaging.