NBD-PE is widely used as a fluorescent lipid probe in biological research to study membrane dynamics and lipid trafficking. Its nitrobenzoxadiazole (NBD) fluorophore allows for easy visualization under fluorescence microscopy, making it ideal for labeling and tracking phospholipids in cellular membranes. Researchers utilize it to investigate lipid distribution, membrane fluidity, and vesicle fusion processes. It is also employed in studying lipid-protein interactions and the behavior of lipid bilayers in model membrane systems. Additionally, NBD-PE is valuable in assays to monitor endocytosis, exocytosis, and intracellular lipid transport pathways. Its compatibility with live-cell imaging makes it a versatile tool for exploring cellular mechanisms involving lipids.