ABD-F is widely used as a derivatizing agent in high-performance liquid chromatography (HPLC) for the sensitive detection and quantification of thiol-containing compounds. It selectively reacts with thiol groups under mild conditions, forming stable fluorescent derivatives that can be easily analyzed. This makes it particularly useful in biochemical and pharmaceutical research for measuring thiols such as glutathione, cysteine, and other sulfhydryl compounds in biological samples. Its high sensitivity and specificity enable accurate determination of thiol levels, which is crucial for studying oxidative stress, redox biology, and drug metabolism. Additionally, ABD-F is employed in environmental analysis to detect thiols in water and other matrices, ensuring precise monitoring of pollutants and contaminants.