DBD-NCS is widely used as a fluorescent labeling reagent in biochemistry and analytical chemistry. It is particularly effective for tagging amino acids, peptides, and proteins due to its reactive isothiocyanate group, which forms stable thiourea bonds with primary amines. This property makes it valuable in high-performance liquid chromatography (HPLC) for the sensitive detection and quantification of biomolecules. Additionally, DBD-NCS is employed in fluorescence spectroscopy and imaging studies, where its strong fluorescence characteristics enhance the visualization and analysis of labeled compounds. Its applications extend to proteomics research, where it aids in the identification and characterization of proteins in complex biological samples.