Used as a fluorescent probe in biochemical and analytical applications, particularly for chiral recognition and detection of biomolecules. Its high fluorescence quantum yield and sensitivity to the local environment make it suitable for labeling amino acids, peptides, and other chiral compounds in HPLC and capillary electrophoresis. The (R)-enantiomer shows selective reactivity and binding preferences in stereospecific assays, enabling precise detection in complex biological samples. Also employed in studies of enzyme activity and inhibition where fluorescence signaling is required.