Used as a fluorescent ceramide analog in cell biology research to study sphingolipid metabolism and membrane trafficking. It labels the Golgi apparatus and endoplasmic reticulum in live cells, allowing visualization of lipid transport pathways. Commonly applied in fluorescence microscopy and flow cytometry due to its bright green emission upon excitation. Also utilized to monitor ceramide conversion to more complex sphingolipids, making it a valuable tool for investigating apoptosis, lipid signaling, and lysosomal storage disorders.