Widely used as a fluorescent labeling reagent in biochemical and cellular assays. Its primary application is in the detection and quantification of biomolecules such as proteins, amines, and amino-containing compounds. The compound reacts selectively with primary amines to form highly fluorescent conjugates, making it ideal for fluorescence-based detection methods including fluorescence microscopy, flow cytometry, and electrophoresis. Commonly employed in labeling peptides and proteins for imaging studies due to its bright green fluorescence and high photostability. Also used in high-sensitivity assays where low detection limits are required, such as in immunoassays and nucleic acid probing. Its water-soluble nature allows for efficient labeling in aqueous buffers, minimizing the need for organic solvents in biological labeling workflows.