2,3-Dihydroxybenzoic acid is primarily used in biochemical research as a chelating agent due to its ability to bind metal ions. It is often employed in studies related to iron metabolism and siderophore activity, helping to understand microbial iron acquisition mechanisms. Additionally, it serves as a precursor in the synthesis of various pharmaceutical compounds, including those with antimicrobial and antioxidant properties. In industrial applications, it is utilized in the production of specialty chemicals and as an intermediate in organic synthesis. Its role in inhibiting certain enzymes also makes it valuable in biochemical assays and enzyme studies.