5-Acetamido-2-nitrobenzoic acid is primarily used in organic synthesis and pharmaceutical research. It serves as a key intermediate in the production of various chemical compounds, particularly in the development of drugs and bioactive molecules. Its structure makes it valuable for creating complex organic frameworks, often utilized in medicinal chemistry for designing potential therapeutic agents. Additionally, it can be employed in biochemical studies to investigate enzyme interactions or as a precursor for dyes and pigments in industrial applications. Its nitro and acetamido functional groups allow for versatile chemical modifications, making it a useful building block in synthetic chemistry.