Tert-butyl 3-nitrobenzoate is primarily used as an intermediate in organic synthesis, particularly in the production of pharmaceuticals and agrochemicals. Its nitro and ester functional groups make it a versatile building block for constructing more complex molecules. In pharmaceutical research, it is often employed in the development of active pharmaceutical ingredients (APIs) due to its ability to introduce nitro and ester moieties into target compounds. Additionally, it serves as a precursor in the synthesis of dyes, pigments, and other specialty chemicals. Its stability and reactivity under various conditions make it a valuable component in laboratory-scale and industrial chemical processes.