Widely used in chemical biology and medicinal chemistry as a highly reactive warhead in covalent inhibitors. Its sulfonyl fluoride group selectively reacts with nucleophilic amino acid residues such as lysine, tyrosine, and serine in proteins, enabling targeted covalent modification. Frequently employed in activity-based protein profiling (ABPP) to identify and characterize enzyme function in complex biological systems. Also utilized in the development of probes for studying protein-ligand interactions due to its stability under physiological conditions and rapid reaction kinetics. Its benzodioxole scaffold enhances membrane permeability, making it suitable for live-cell labeling applications. Increasingly applied in drug discovery for irreversible inhibition of disease-relevant enzymes.