PIPES Buffered Saline is widely used in biological and biochemical research due to its ability to maintain a stable pH in cell culture and experimental conditions. It is particularly effective in buffering solutions within the pH range of 6.1 to 7.5, making it suitable for applications involving sensitive biological materials. This buffer is commonly employed in cell lysis procedures, protein purification, and electrophoresis, where consistent pH is critical for maintaining protein stability and function. Additionally, PIPES Buffered Saline is often used in fluorescence microscopy and other imaging techniques, as it does not interfere with fluorescent dyes or indicators. Its low reactivity with metal ions also makes it a preferred choice in studies involving metal-dependent enzymes or processes. Overall, it is a versatile buffer solution that supports a wide range of laboratory applications requiring precise pH control.