Krebs-Henseleit solution is widely used in biomedical research, particularly in studies involving isolated organs or tissues. It serves as a physiological buffer to maintain the viability and functionality of tissues during experiments. Commonly applied in perfusing organs like the liver, heart, and kidneys, it mimics the ionic composition of blood, ensuring stable pH and osmotic balance. This solution is essential in metabolic studies, drug testing, and toxicity assessments, as it supports normal cellular respiration and metabolic processes. Its role in maintaining tissue integrity makes it a critical tool in understanding organ function and response to various stimuli under controlled conditions.