HEPES Buffered Saline is widely used in biological and biochemical research due to its ability to maintain a stable pH in cell culture environments. It is commonly employed in cell and tissue culture applications, providing an optimal environment for cell growth and experimental consistency. The buffer is also utilized in various molecular biology techniques, such as protein purification, electrophoresis, and immunostaining, where pH stability is critical. Additionally, HEPES Buffered Saline is often used in fluorescence microscopy and other imaging techniques to ensure minimal pH fluctuations that could affect the accuracy of results. Its compatibility with live cells and enzymes makes it a preferred choice in many laboratory protocols.