HEPES Buffered Saline is widely used in biological and biochemical research due to its ability to maintain a stable pH in cell cultures and experiments. It is particularly valuable in cell and tissue culture applications, where it provides a consistent environment for cell growth and viability. The buffer is also utilized in protein studies, enzyme assays, and molecular biology techniques, as it minimizes pH fluctuations that could affect experimental outcomes. Additionally, HEPES Buffered Saline is employed in microscopy and imaging studies to maintain cell integrity during observation. Its compatibility with various biological systems makes it a preferred choice for maintaining physiological conditions in vitro.