HEPES Buffered Krebs-Ringer Solution is widely used in biological and medical research due to its ability to maintain a stable pH in cell cultures and physiological experiments. It is particularly valuable in studies involving cell metabolism, as it provides a balanced ionic environment similar to that of bodily fluids. This solution is often employed in experiments requiring long-term cell viability, such as tissue perfusion, organ preservation, and in vitro fertilization. Its buffering capacity ensures minimal pH fluctuations, making it ideal for sensitive biochemical assays and electrophysiological studies. Additionally, it is used in drug screening and toxicity testing to simulate physiological conditions accurately.