Dulbecco's phosphate buffer premixed powder is widely used in biological and biochemical research for maintaining pH stability (7.2-7.6 when reconstituted) in cell cultures and experiments. Free of calcium, magnesium ions, and phenol red, it provides an isotonic environment, making it ideal for washing cells, diluting reagents, and preparing media. This buffer is also commonly employed in immunology and histochemistry for antibody staining and tissue sample preparation. Its balanced composition ensures minimal disruption to cellular functions, making it a reliable choice for in vitro studies. Additionally, it is used in diagnostic assays and as a rinse solution in laboratory protocols to maintain consistent conditions.