HEPES Buffered Saline is widely used in biological and biochemical research due to its ability to maintain a stable pH environment. It is commonly employed in cell culture applications to provide optimal conditions for cell growth and viability. The buffer is particularly useful in experiments involving live cells, as it minimizes pH fluctuations caused by metabolic processes. It is also utilized in protein studies, including electrophoresis and chromatography, where maintaining a consistent pH is critical for accurate results. Additionally, HEPES Buffered Saline is often used in immunoassays and fluorescence microscopy to ensure stable conditions during sample preparation and analysis. Its compatibility with various biological systems makes it a versatile choice for a range of laboratory procedures.