Amphibian Ringer Solution is primarily used in biological and physiological research involving amphibians. It serves as an isotonic solution that mimics the natural extracellular fluid environment, ensuring the viability of tissues and organs during experiments. This solution is essential for maintaining the physiological conditions of amphibian cells, tissues, or organs in vitro, such as in studies involving muscle contraction, nerve conduction, or heart function. It is also employed in electrophysiology experiments to keep amphibian specimens alive and functional for extended periods. Additionally, it is used in teaching laboratories to demonstrate fundamental physiological principles in amphibian models, such as frogs. Its balanced ionic composition supports the integrity of cellular structures and ensures accurate experimental results.