Ribocil B is primarily used in research to study bacterial riboflavin biosynthesis pathways. It acts as a potent inhibitor of riboflavin production in bacteria by binding to the FMN riboswitch, which regulates the expression of genes involved in riboflavin synthesis. This makes it a valuable tool for understanding bacterial metabolism, riboswitch mechanisms, and developing novel antibacterial strategies. By targeting riboflavin synthesis, it helps identify potential drug targets for combating bacterial infections, particularly in antibiotic-resistant strains. Its application extends to exploring bacterial survival mechanisms and evaluating the efficacy of new antimicrobial agents.