Used historically in research as a dye intermediate and model compound in studies of azo compound metabolism. It has been employed in biochemical assays to investigate liver enzyme activity, particularly in relation to azo dye detoxification pathways. Due to structural properties, it served as a reference substance in toxicology studies examining the effects of aromatic amines and chlorinated azo compounds on cellular systems. Its application is primarily limited to laboratory research, with no significant industrial or commercial use in modern contexts. Caution is advised due to potential toxicity and carcinogenicity with prolonged exposure.