This chemical is widely used in bioconjugation and click chemistry applications. Its azide group allows for efficient and selective reactions with alkyne-containing molecules, making it valuable for labeling biomolecules such as proteins, peptides, and nucleic acids. The NHS ester group enables covalent attachment to primary amines, facilitating the modification of proteins or other amine-containing compounds. The PEG3 spacers enhance solubility and reduce steric hindrance, improving the efficiency of conjugation processes. The central N-Boc group acts as a protecting group for the amine linkage, which can be deprotected under mild acidic conditions to allow for further functionalization. This compound is particularly useful in drug delivery systems, where it can be used to create targeted therapies by linking drugs to antibodies or other targeting agents. Additionally, it is employed in the development of biophysical probes for studying molecular interactions and cellular processes. Its versatility and reactivity make it a key tool in chemical biology and materials science research.