This compound is widely used in bioconjugation processes, particularly for attaching biomolecules such as proteins, peptides, or antibodies to other molecules or surfaces. Its NHS ester group reacts efficiently with primary amines, forming stable amide bonds, while the azido group enables further modification through click chemistry, such as copper-catalyzed azide-alkyne cycloaddition (CuAAC). This makes it a valuable tool in drug delivery systems, where it helps create targeted therapeutic agents by linking drugs to carriers like nanoparticles or antibodies. Additionally, it is employed in the development of biosensors and diagnostic assays, enabling the precise attachment of detection molecules to substrates. Its PEG spacer enhances solubility and reduces steric hindrance, improving the efficiency of conjugation reactions.