This chemical is widely used in bioconjugation processes, where it serves as a linker to attach biomolecules such as proteins, peptides, or antibodies to other entities like surfaces, nanoparticles, or polymers. The azide group allows for click chemistry reactions, particularly copper-catalyzed azide-alkyne cycloaddition (CuAAC), enabling efficient and specific coupling. The NHS ester group reacts with primary amines, facilitating stable amide bond formation with target molecules. Its PEG12 spacer provides flexibility and solubility, reducing steric hindrance and improving the overall efficiency of the conjugation process. This makes it valuable in drug delivery systems, diagnostic assays, and the development of bioconjugated materials for research and therapeutic applications.