This compound is widely used in bioconjugation applications, particularly for attaching biomolecules such as proteins, peptides, or antibodies to other molecules or surfaces. Its NHS ester group reacts efficiently with primary amines, forming stable amide bonds, while the azido group allows for further functionalization via click chemistry, such as copper-catalyzed azide-alkyne cycloaddition (CuAAC). This makes it a valuable tool in the development of drug delivery systems, diagnostic probes, and biomaterial modifications. Its PEG spacer enhances solubility and reduces steric hindrance, improving the efficiency of conjugation reactions.