Used in bioconjugation, surface functionalization, and biomolecular labeling for biosensor development and diagnostic applications. The biotin moiety enables specific and high-affinity binding to streptavidin or avidin, facilitating detection, purification, and immobilization of target biomolecules such as proteins or nucleic acids. The picolyl azide group supports efficient copper-free click chemistry (strain-promoted azide-alkyne cycloaddition, SPAAC) with strained alkynes like DBCO, enabling selective labeling under mild, biocompatible conditions without copper toxicity. The PEG4 spacer improves water solubility, flexibility, and reduces non-specific interactions, enhancing signal-to-noise ratios in assays. The dialkoxydiphenylsilane moiety provides stable covalent attachment to silica-based surfaces, including glass slides, silicon wafers, and nanoparticles, for creating bioactive interfaces in microarrays, lab-on-a-chip systems, and targeted delivery platforms. Commonly applied in proteomics, bioimaging, biosensors, and precise surface modification techniques.