Used in bioconjugation and labeling applications due to its azide group, which readily participates in click chemistry reactions, especially copper-catalyzed azide-alkyne cycloaddition (CuAAC). The hydroxycoumarin moiety provides fluorescent properties, making it useful as a fluorescent probe in biological imaging and detection. Commonly employed in the development of sensors, fluorescent tags for biomolecules, and in studying cellular processes through fluorescence microscopy. Its reactivity and optical characteristics also support its use in photolabeling and photochemical studies.