Used in click chemistry reactions, particularly via copper-catalyzed azide-alkyne cycloaddition (CuAAC), due to the azide group, enabling efficient bioconjugation and formation of stable triazole linkers for biomolecules such as proteins, enzymes, or bioactive agents with vectors, nanoparticles, or imaging probes.

Its polyether chain provides water solubility and flexibility, reducing non-specific protein binding, making it suitable for modifying peptides, proteins, and nanoparticles in targeted drug delivery and diagnostic applications, including specific cell labeling in research.

The 2-fluoropyridine moiety enhances metabolic stability and serves as a lead structure for designing new bioactive compounds. Commonly employed in the development of bioorthogonal probes for imaging and targeted therapy.