This compound is widely used in bioconjugation processes, particularly for attaching biomolecules like proteins, peptides, or antibodies to other entities such as polymers, surfaces, or nanoparticles. The NHS ester group reacts efficiently with primary amines, enabling stable amide bond formation, while the azido group allows for further functionalization through click chemistry, such as copper-catalyzed azide-alkyne cycloaddition (CuAAC). This dual functionality makes it valuable in drug delivery systems, diagnostic assays, and the development of biomaterials. Its PEG spacer enhances solubility and reduces steric hindrance, improving the efficiency of conjugation reactions in aqueous environments.